Guto Montezano @guto_montezano
I am a scientist in Cardiovascular Medicine @RIMUHC1 My goal: to pay it forward and share the research of all of us for better lives! Montreal, CA @RI-MUHC1 Joined January 2017-
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@guto_montezano @ashleylpitzer @annetkiraboc1 Thank you so much for hosting this wonderful session @guto_montezano and thanks to everyone for all the great questions! @HyperAHA #HYPHIP
Answer to @DrDudenbostel #HYPHIP
@guto_montezano @fjorios @ashleylpitzer @LErtuglu Q12 - in our initial paper published in @jci we performed a comprehensive analysis and found IsoLgs as extremely potent compared to other lipid mediators #HYPHIP @HyperAHA
@fjorios @annetkiraboc1 @ashleylpitzer @HyperAHA @guto_montezano A12 Yes both innate and adaptive immunity appear to play key roles! This is a very interesting point to look into in future studies! @fjorios @HyperAHA #HYPHIP
@guto_montezano @ashleylpitzer @LErtuglu @HyperAHA A15 We have not tested K. Hypothesis it would be protective but we need to study this #HYPHIP @HyperAHA
Wow how time flies by when you are doing something interesting and fun! The end of our chat has arrived, sadly, because I was learning a lot and really enjoying talking to @ashleylpitzer @annetkiraboc1 @LErtuglu Thank you so much to you all for being here with us today! #HYPHIP
@annetkiraboc1 @guto_montezano @ashleylpitzer @HyperAHA In our previous study using CITE-sequence analysis, we found no significant change in the expression of any RAAS-related gene in APCs after salt loading and depletion. @HyperAHA #HYPHIP @guto_montezano frontiersin.org/journals/physi…
Q12 from @fjorios to @ashleylpitzer @annetkiraboc1 @LErtuglu may have be lost in the middle of the chat
Q12 from @fjorios to @ashleylpitzer @annetkiraboc1 @LErtuglu may have be lost in the middle of the chat
@guto_montezano @ashleylpitzer @LErtuglu @HyperAHA A14 salt-sensitive people have super sensitive and high expression of SGK1. Our data indicates not through the MR. It’s the GR. Aldo does not correlate with salt-sensitive hypertension #HYPHIP @HyperAHA
@guto_montezano @ashleylpitzer @LErtuglu @HyperAHA A13. Yes indeed. We find vascular dysfunction plays a role. Endothelial cells produce VCAM, ICAM that attract immune cells, which then gain access to tissues #HYPHIP @HyperAHA
@guto_montezano @ashleylpitzer @annetkiraboc1 @HyperAHA A13 2/2 While we could not assess vascular function in the current study, previously @CVimmunology @endocelldoc found that T-cell activation and IL-17A production lead to vascular dysfunction by altering vascular reactivity and increasing vascular superoxide @HyperAHA #HYPHIP
Q15 @ashleylpitzer @annetkiraboc1 @LErtuglu With the rise of K as a Na substitute, what would you expect if you used K-enriched salt? #HYPHIP @HyperAHA
@guto_montezano @ashleylpitzer @annetkiraboc1 @HyperAHA A13 1/2 This is a great question. Dr. Laffer and colleagues previously showed that salt sensitivity is associated with increased vascular resistance, suggesting that vascular dysfunction plays a key role in salt sensitivity. @HyperAHA #HYPHIP
Q14 @ashleylpitzer @annetkiraboc1 @LErtuglu In your data, you didn't see a role for aldosterone. Do you know if in isolevuglandin-activated APCs, MR activation is increased? And What about cortisol? #HYPHIP @HyperAHA
@DrDudenbostel @guto_montezano @HyperAHA @annetkiraboc1 @ashleylpitzer A11 Thank you for this important Q, @DrDudenbostel! Previous studies showed that women are affected by SS more. We are studying possible sex differences in immune-cell salt sensitivity in ongoing studies! @HyperAHA #HYPHIP pubmed.ncbi.nlm.nih.gov/34569287/
A11 2/2 #HYPHIP
A11 #HYPHIP
Q13 @ashleylpitzer @annetkiraboc1 @LErtuglu You have seen an association between IsoLv activation of APCs and pro-inflammatory cytokines in salt-sensitivity. Are these changes are associated with changes in vascular function or structure in your cohort? #HYPHIP @HyperAHA
@annetkiraboc1 @ashleylpitzer @LErtuglu @HyperAHA Q12. A mostly innate immune mechanisms them? Very interesting. I wonder whether other lipid mediators might also be involved? @ashleylpitzer @annetkiraboc1 @LErtuglu #HYPHIP @HyperAHA @guto_montezano
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I am thrilled to announce the acceptance of my 1st author publication examining maternal behavior & brain inflammation. An abundance of gratitude to my amazing mentors @SGoulopoulou and @RebecCunningham for supporting my independent research in #maternalbrain health and disease.
🌟New Publication🌟 The team is on a roll - Our paper on #maternal brain 🧠 inflammation during healthy #pregnancy is accepted! Congrats 🎉 to rising star ⭐️ @DrJLBradshaw and co-authors and cheers to our collaborator @RebecCunningham #maternalhealthresearch
✨New Publication✨ Our review paper 📝 on perivascular adipose tissue and uterine artery blood flow was accepted for publication in #Microcirculation - Congrats to Deborah Osikoya and @reneeooliveira @hula_nataliia #vascular #pregnancy #MaternalHealth
@guto_montezano @ashleylpitzer @annetkiraboc1 Thank you so much for hosting this wonderful session @guto_montezano and thanks to everyone for all the great questions! @HyperAHA #HYPHIP
@DrDudenbostel @guto_montezano @ashleylpitzer @annetkiraboc1 @HyperAHA @CVimmunology @endocelldoc We did not see a difference in baseline pulse pressure between salt-sensitivity tertiles, but pulse pressure significantly decreased from salt-loading to depletion in salt-sensitive people compared to salt-resistant @HyperAHA #HYPHIP
@guto_montezano @fjorios @ashleylpitzer @LErtuglu Q12 - in our initial paper published in @jci we performed a comprehensive analysis and found IsoLgs as extremely potent compared to other lipid mediators #HYPHIP @HyperAHA
@fjorios @annetkiraboc1 @ashleylpitzer @HyperAHA @guto_montezano A12 Yes both innate and adaptive immunity appear to play key roles! This is a very interesting point to look into in future studies! @fjorios @HyperAHA #HYPHIP
@guto_montezano @ashleylpitzer @LErtuglu @HyperAHA A15 We have not tested K. Hypothesis it would be protective but we need to study this #HYPHIP @HyperAHA
@annetkiraboc1 @guto_montezano @ashleylpitzer @HyperAHA In our previous study using CITE-sequence analysis, we found no significant change in the expression of any RAAS-related gene in APCs after salt loading and depletion. @HyperAHA #HYPHIP @guto_montezano frontiersin.org/journals/physi…
@LErtuglu @guto_montezano @ashleylpitzer @annetkiraboc1 @HyperAHA @CVimmunology @endocelldoc Did see differences in pulse pressure as a crude estimate of arterial stiffness? #hyphip @guto_montezano @HyperAHA @LErtuglu @annetkiraboc1
@guto_montezano @ashleylpitzer @LErtuglu @HyperAHA A14 salt-sensitive people have super sensitive and high expression of SGK1. Our data indicates not through the MR. It’s the GR. Aldo does not correlate with salt-sensitive hypertension #HYPHIP @HyperAHA
@guto_montezano @ashleylpitzer @LErtuglu @HyperAHA A13. Yes indeed. We find vascular dysfunction plays a role. Endothelial cells produce VCAM, ICAM that attract immune cells, which then gain access to tissues #HYPHIP @HyperAHA
@guto_montezano @ashleylpitzer @annetkiraboc1 @HyperAHA A13 2/2 While we could not assess vascular function in the current study, previously @CVimmunology @endocelldoc found that T-cell activation and IL-17A production lead to vascular dysfunction by altering vascular reactivity and increasing vascular superoxide @HyperAHA #HYPHIP
@guto_montezano @ashleylpitzer @annetkiraboc1 @HyperAHA A13 1/2 This is a great question. Dr. Laffer and colleagues previously showed that salt sensitivity is associated with increased vascular resistance, suggesting that vascular dysfunction plays a key role in salt sensitivity. @HyperAHA #HYPHIP
@DrDudenbostel @guto_montezano @HyperAHA @annetkiraboc1 @ashleylpitzer A11 Thank you for this important Q, @DrDudenbostel! Previous studies showed that women are affected by SS more. We are studying possible sex differences in immune-cell salt sensitivity in ongoing studies! @HyperAHA #HYPHIP pubmed.ncbi.nlm.nih.gov/34569287/
@DrDudenbostel @guto_montezano @HyperAHA @ashleylpitzer More in women even in pre-menopause but amplified by menopause
@DrDudenbostel @guto_montezano @HyperAHA @ashleylpitzer We are not yet powered to answer this for immune mechanisms - it is ongoing but all large clinical trials that have rigorously phenotyped salt-sensitivity find it mainly in women
@annetkiraboc1 @ashleylpitzer @LErtuglu @HyperAHA Q12. A mostly innate immune mechanisms them? Very interesting. I wonder whether other lipid mediators might also be involved? @ashleylpitzer @annetkiraboc1 @LErtuglu #HYPHIP @HyperAHA @guto_montezano
@BuchanMigd70994 @fjorios @ashleylpitzer @LErtuglu @HyperAHA @guto_montezano To clarify, in our paper we think EETs regulating APC ENaC are coming from the kidney. Not sure what specific cell type in the kidney but endothelial cells could be a source #HYPHIP @HyperAHA
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