News from the Sternberg lab at Columbia Univ., HHMI. Posts are from lab members and not Samuel Sternberg unless signed SHS. Posts represent personal views only.sternberglab.org New York, NYJoined October 2017
8/8 Want more? The preprint has it all-- subcomplex structures, mutagenesis validations & a clear path toward engineering next-gen gene insertion tools. Huge congrats to co-first authors Giada and Seraina, and the whole Jinek & Sternberg lab teams! 🎉 tinyurl.com/typeI-CAST
1/8 🚨 New preprint from the @SternbergLab & Jinek labs! CRISPR-associated transposases (CASTs) insert large DNA cargoes at precise genomic locations — no double-strand breaks needed.
9/9 This was a huge team effort! Big thanks to Kanta, Junichiro, and the Nishimasu lab for adjusting their meeting schedules to collaborate across time zones. Equal gratitude goes out to the Sternberg lab for the discussions and shared curiosity that shaped this work!
1/9 New preprint from the Sternberg Lab in collaboration with the Nishimasu Lab! We uncover how the DRT3 antiphage immune system pairs two reverse transcriptases, one RNA-templated and one protein-templated, to build a double-stranded DNA effector. doi.org/10.64898/2026.…
In summary: IStrons cram three mutually competing functions into one sequence. Our library approach reveals the molecular rules governing this balance, and why ωRNA consistently comes out on top.
biorxiv.org/content/10.648…
Splicing has an Achilles' heel: it's exquisitely sensitive to 3' exon sequences. Each new transposition event generates a novel exon context — many incompatible with efficient splicing. This vulnerability may explain why ωRNA formation is so heavily favored.
New preprint from the Sternberg lab!
biorxiv.org/content/10.648…
We used pooled oligonucleotide library mutagenesis + high-throughput sequencing to systematically dissect the molecular determinants of IStron transposition, RNA-guided DNA cleavage, and self-splicing.
884 Followers 938 FollowingInterested in molecular plasticity of both first and second brains. Incoming faculty member @TIFR_Bio Mumbai. Currently in https://t.co/M939WRg3W2 @hhmi_science @Columbia
2K Followers 2K Following@CFWenceslau Laboratory of Vascular Biology - LVB University of South Carolina School of Medicine @UofSCSOMC @CTRC_UofSCSOM 👩🔬❤️ 🧫Tweets =mine
1K Followers 3K FollowingInterested in how cells form complex structures. Postdoc studying elegant cell-cell connections. Former dicer of planarians. Musings are my own.
271 Followers 2K FollowingPostDoc @MountSinaiNYC Research on 3neg breast cancer. Political Junkie, ⚽️ Enthusiast, and rookie birdwatcher👩🏽🔬 🦉 Thoughts are my own.
350 Followers 286 FollowingPhD student at @AzeredoLab, University of Minho (Braga, Portugal).
Interested in phages, microbiology and genetic engineering.
522 Followers 2K Following#MolecularBiology @riceuniversity , Employing #Zebrafish to understand #devbio and #cancer. In awe with life through #science. A trascending #millenial.
73K Followers 13K FollowingI science & try to make science open. Tweets do not represent my employer's views, but are always correct. My conflicts of interest https://t.co/77P0LDe6VR.
5K Followers 1K FollowingAssistant Professor at the University of Zurich. 🖥️ protein design, machine learning🤖, crystallography💎, cryoEM🔬. Avid weirdness connoisseur 🎩
338 Followers 394 FollowingIncoming Assistant Professor at UCLA • #BWFPDEP Postdoc @Columbia • @SFSU @JohnsHopkins alumni • Archaea fan • Metagenomic Editing of the gut and beyond ✂️🧬🦠
79K Followers 68 FollowingProfessor at Harvard and the Broad Institute. Our lab integrates chemistry & evolution to illuminate biology & enable new therapeutics. IG/Threads: @davidrliu1
759 Followers 749 Following@UCB_Chemistry PhD Candidate @schepartzlab/@cgem_cci 🐻 | @NUChemistry & @MirkinGroupNU Alum | 🇵🇷🇭🇰 | also @alexsolivan on the other place 🟦☁️
536 Followers 496 FollowingPostdoc at Wiedenheft Lab 🥼 | Former HFSP postdoctoral fellow at Sternberg Lab| 🧬 CRISPR-Cas enthusiast | Interested in mobile genetic elements
23K Followers 2K FollowingMicrobiology, evolution, antibiotic resistance, applied math, molecular biotech. Associate Professor @HarvardMed. Basic research is the engine of progress
2K Followers 711 FollowingDean of Science, Professor of Chemistry, and Professor of Physiology & Cellular Biophysics at Columbia University | Championing Science, Learning, and Community