Sternberg Lab @SternbergLab

News from the Sternberg lab at Columbia Univ., HHMI. Posts are from lab members and not Samuel Sternberg unless signed SHS. Posts represent personal views only. sternberglab.org New York, NY Joined October 2017

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Sternberg Lab @SternbergLab

2 weeks ago

8/8 Want more? The preprint has it all-- subcomplex structures, mutagenesis validations & a clear path toward engineering next-gen gene insertion tools. Huge congrats to co-first authors Giada and Seraina, and the whole Jinek & Sternberg lab teams! 🎉 tinyurl.com/typeI-CAST

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Sternberg Lab @SternbergLab

2 weeks ago

7/8 The full mechanistic model 🏛️: every step gated, every checkpoint structural. A complete blueprint for engineering better gene insertion tools. 🛠️

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Sternberg Lab @SternbergLab

2 weeks ago

1/8 🚨 New preprint from the @SternbergLab & Jinek labs! CRISPR-associated transposases (CASTs) insert large DNA cargoes at precise genomic locations — no double-strand breaks needed.

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Sternberg Lab @SternbergLab

3 weeks ago

Be sure to also check out the elegant concurrent work from Alex Gao's group: science.org/doi/10.1126/sc…

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Sternberg Lab @SternbergLab

3 weeks ago

9/9 This was a huge team effort! Big thanks to Kanta, Junichiro, and the Nishimasu lab for adjusting their meeting schedules to collaborate across time zones. Equal gratitude goes out to the Sternberg lab for the discussions and shared curiosity that shaped this work!

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Sternberg Lab @SternbergLab

3 weeks ago

1/9 New preprint from the Sternberg Lab in collaboration with the Nishimasu Lab! We uncover how the DRT3 antiphage immune system pairs two reverse transcriptases, one RNA-templated and one protein-templated, to build a double-stranded DNA effector. doi.org/10.64898/2026.…

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Sternberg Lab @SternbergLab

3 months ago

In summary: IStrons cram three mutually competing functions into one sequence. Our library approach reveals the molecular rules governing this balance, and why ωRNA consistently comes out on top. biorxiv.org/content/10.648…

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Sternberg Lab @SternbergLab

3 months ago

Splicing has an Achilles' heel: it's exquisitely sensitive to 3' exon sequences. Each new transposition event generates a novel exon context — many incompatible with efficient splicing. This vulnerability may explain why ωRNA formation is so heavily favored.

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Sternberg Lab @SternbergLab

3 months ago

New preprint from the Sternberg lab! biorxiv.org/content/10.648… We used pooled oligonucleotide library mutagenesis + high-throughput sequencing to systematically dissect the molecular determinants of IStron transposition, RNA-guided DNA cleavage, and self-splicing.