Josef Lazar @LazarLab
Seeing new things by using fluorescent proteins and interesting microscopes Joined December 2020-
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@JohannesPilic My subjective perception of scientific success... A little hard to measure accurately🙂 My postdoctoral lab running out of money before I published anything was hard
Unrelated: last week I gave a seminar for the students of the Science program of Charles University (natur.cuni.cz/uchazeci/studi…). Seems like an excellent program and a lovely group of students!👍😍 I hope they enjoyed it as much as I did!
I took my hand (specifically, my hurting middle finger) to the doctor today... Here is the result😇. They made me do it😂. Less fun: if you look closely, you will see that the end of the bone is crushed🙄
Got my hand pinched by a door a week ago... Ouch! 😰 One of my fingers still hurts, but Petro has now at least made it look better... At least in Photoshop🙂
The actual video can be watched here... We are on at time 6:29 ct24.ceskatelevize.cz/rubrika/veda-2…
Our HEK293 cells celebrate Valentine's day! ♥️🙂
Check out how this translocation-based biosensor develops linear dichroism (when the orientational freedom of the fluorescent protein becomes restricted)! 😎 Imaged with our new, super-fast confocal LD microscope🔬⚡️, now available from innovativebioimaging.com
We used a super-fast linear dichroism confocal microscope based developed in collaboration with our friends at KU Leuven: The TriScan: fast and sensitive 3D confocal fluorescence imaging using a simple optical design biorxiv.org/content/10.110…
Microscopy imaging of activation of the b2-adrenergic receptor by using a FLIP biosensor - resting state (on the left) and after b2AR activation (on the right). Can you spot the difference?🤔 Pretty cool, huh? 😎🙂
FLIPs are new, super-simple molecular biosensors that convert molecular events into changes in orientation of a fluorescent protein. This change is then observable by linear dichroism (LD) microscopy. Isn't this simple?
And this is the paper we were celebrating!🥂: FLIPs: Genetically encoded molecular biosensors for functional imaging of cell signaling by linear dichroism microscopy | Science Advances science.org/doi/10.1126/sc…
We had a little celebration of our upcoming paper in Science Advances... One of the co-authors showed up in full strength!💪😱 Keep up the good work, Alex!😂
Electronics is fun! I did some microscopic soldering for the first time today...🔬😎 It worked!💪🏼
We see that an intrabody based on NB32 distinguishes between different GPCR conformations. This can be used in biosensors based on bioluminescence, but also in fluorescent biosensors that utilize linear dichroism/polarization microscopy🔬. Check it out!
After many, many days and nights of work, our joint paper with the Shukla lab (@arshukla) is out: pnas.org/doi/10.1073/pn…
A joint manuscript with @arshukla accepted, with Vendy Nagy Marková as a first co-author! Thank you, Arun & al.! Btw., first submission: 1.5 years ago 😂
Got back from the EMC meeting in Copenhagen @eurmicsoc #EMC2024... Impressive results by @CizmarTomas, and a well-deserved prize, for imaging through an optical fiber🙎♂️🧵🔎🎥🖼️! Also impressive: the Copenhagen airport security!👮😉
@MGMlab Well, the rest of us were tired the next day 😂 Anyway - glad you could make it!
Another summer visitor to the lab: Mikel Garcia-Marcos (@MGMlab) from Boston U. stayed for a whole month, and even came to our lab retreat!🏖️🍹🥤🍺 🍲🌭Excellent exchange of know-how! Curious to see what comes out of it! 💡🔬
Finally: we are now revising our joint paper with Arun Shukla (@arshukla) on cool conformation biosensors of arrestin signaling: biorxiv.org/content/10.110… Looking forward to getting it published!
Bims: Biomed News @Bims_BiomedNews
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Alejandra Tomás Cata... @TomasLab_ICL
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Mikel Garcia-Marcos L... @MGMlab
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Ivan Maslov @IvanMasl0v
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Nathan Shaner 🏳️... @NathanShaner
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ÚEB AV ČR @UEBavcr
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Rita Strack @rita_strack
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Maria del Mar Ingaram... @Maria_Ingaramo
852 Followers 782 Following I'm a biochemist. I develop new fluorescent proteins and biosensors.
Katarina Nemec @karantarina
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Tomas Moravec 🇨�... @moravec_tomas
1K Followers 3K Following Plant virologist, environmentalist, dad 👨🔬👨🌾👱♂️👨🏫🤦♂️👨👩👧👦🦠🌱🍀🍎🍌🌽🥬🍅🍆🎾🏒🚴♂️🚗🚃⛺️📷🖥️🛠️🧬🧬🔬🧪🧫💉⚗️⚛️☣️🏴☠️🇨🇿
Jennifer Waters @JenCWaters
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Quantitative Imaging @QIatCSHL
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Eva Bártová - Cell ... @EvaIbp
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FocalPlane @focalplane_jcs
6K Followers 513 Following FocalPlane is a community site for anyone with an interest in microscopy. Hosted by @J_Cell_Sci and @Co_Biologists.
Brandán Pedre @Brandanium
288 Followers 422 Following I make fluorescent indicators that dynamically monitor metabolites and signaling molecules in the cell. Now @KULeuven, previously in @DKFZ and @VIBLifeSciences.
DrGPCR @DrGPCR
4K Followers 4K Following Join the movement, connect, share, and speed GPCR drug discovery. #GPCR Ecosystem. https://t.co/jPzcdANDn1
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Franziska Bierbuesse @FBierbuesse
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Jaroslava Seflova @JaroslavaNKA
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Biophysical Society @BiophysicalSoc
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