qbase+ @qbaseplus

Helping researchers around the world to analyze qPCR data faster. qbaseplus.com Ghent, Belgium Joined February 2016

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qbase+ @qbaseplus

8 years ago

@Erinc2537 Hi Eric, just send a short mail to [email protected] and we'll send you the address.

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qbase+ @qbaseplus

8 years ago

qbase+? That's quality control, normalization, interpretation and storage of your qPCR data. All in one application. Guaranteed worry-free! bgzlle.com/2PYhzyM

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qbase+ @qbaseplus

8 years ago

Improve the quality of standard curves by adding carrier (e.g. tRNA from brewer's yeast) to each dilution point. #qPCR #tip of the day

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qbase+ @qbaseplus

8 years ago

Deliver high quality qPCR results without losing yourself in complex formulas for normalization. Rely on our expertise that comes with a qbase+ subscription! bgzlle.com/2Q5jDp1

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qbase+ @qbaseplus

8 years ago

Not all commercially available qPCR assays have been validated in the lab; make sure you have access to (your own) validation data #qPCR #tip of the day

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The median Cq value is a robust average, and obviates the need for PCR replicate outlier removal. qbase+ allows you to choose between median or arithmetic mean to calculate average Cq value. bgzlle.com/2MWUY3W

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qbase+ @qbaseplus

8 years ago

How many qPCR replicates? 1 is ok in large screening where power comes from sample size and individual errors do not affect the outcome bgzlle.com/2O7RUq6

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qbase+ @qbaseplus

8 years ago

Adhering to the MIQE guidelines improves your qPCR results and facilitates publication. bgzlle.com/2xAnzXY

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qbase+ @qbaseplus

8 years ago

Keep your budget under control by doing qPCR in 15 µl reactions (96-well) or 10 µl reactions (384-well). #qPCR #tip of the day

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qbase+ @qbaseplus

8 years ago

A final concentration of 250 nM of each #qPCR primer is a good compromise between efficiency, yield and specificity. #qPCR #tip of the day

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qbase+ @qbaseplus

8 years ago

Prefer more biological replicates over more technical replicates when trying to prove research questions. bgzlle.com/2Q09JVk

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qbase+ @qbaseplus

8 years ago

A qbase+ subscription brings return on investment: you spend less budget on qPCR repeats and less time in qPCR data normalization! Learn more at bgzlle.com/2Q09F82

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qbase+ @qbaseplus

8 years ago

If you have three genes that fit in two runs, do the measurements in three runs to avoid inter-run variation. #qPCR #tip of the day

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qbase+ @qbaseplus

8 years ago

With qbase+ default settings, even your mom can analyze your qPCR data! bgzlle.com/2xzUaNn

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qbase+ @qbaseplus

8 years ago

Use well-calibrated pipets and avoid pipeting volumes smaller than 2 µl. bgzlle.com/2Q0IHx5

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qbase+ @qbaseplus

8 years ago

Go for more simple and efficient qPCR normalization with qbase+. Learn more at bgzlle.com/2Q0DW6F

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qbase+ @qbaseplus

8 years ago

Prevalidate your reference genes for expression stability before using them in your specific experiment. More information on qPCR normalization on The Gene Expression Blog bgzlle.com/2xHcwML