Nagarjuna Nagaraj @nagarjunanagara
old fashioned protein biochemist but also MS and OMICS crazy Munich, Bavaria Joined October 2015-
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@UCDProteomics @vanaernum but someone should take the responsibility to define a limit below which it should be illegal to call that as gradient. It should be called abruptient.😂
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Any suggestions on how to get pseudo mgf out of regular diaPASEF data?? Thanks.
@UCDProteomics There is still use for DDA and i personally prefer running DDA to get a Preview into the data using Protein metrics Preview. and hopefully i don't get arrested for saying the following 😟- there is still use for in-gel proteomics in some cases despite it being out of fashion
@DemichevLab @BrukerMassSpec Important is to mention - Slice-PASEF can be done on the current timsTOF platform and readily accessible to anyone running a commercially available instrument. Shows the TRUE POWER of DIA-NN.
@DemichevLab @BrukerMassSpec Amazing!!while working 4 timsTOF SCP wanted 2 demonstrate sensitivity boost; sadly couldnt process these data on MQSpectronautDIA-NN then. could be finally processed almost after 2 years :) back then Vadim indicated it is in pipeline. Now accessible- BIG thanks 2 Vadim et al.
@AlexHgO How was the comparison of XC vs C18??
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