Addgene @Addgene
Sharing speeds science! Addgene helps scientists around the world find and share research materials - plasmids, viral preps, antibodies, data, and more. addgene.org Watertown, MA Joined October 2009-
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🎊 Happy birthday, @Addgene! 🥳 Today we cell-ebrate you and the 137,289 plasmids you have sent on behalf of 5,822 labs from around the world!🤯 Get to know Addgene's #OpenScience mission: addgene.org
Our Cell Line of the Week is SERCA2 - illuminating the sarcoplasmic/ endoplasmic reticulum! 💪 Browse our openly available hiPSC lines & plasmids – fluorescently-tagged to illuminate structures: bit.ly/AccessAllenCel… 📦 Distributed by @Coriell_Science & @Addgene
The key reagents from our recent CombiCell manuscript are now available on @Addgene addgene.org/Omer_Dushek/ @embojournal embopress.org/doi/full/10.10…
The key reagents from our recent CombiCell manuscript are now available on @Addgene addgene.org/Omer_Dushek/ @embojournal embopress.org/doi/full/10.10…
Working with @Addgene to make all plasmids easy to get soon
Our #CellLineOfTheWeek is ras-related protein - targeting endosomes! 🧫 Our hiPSC lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCel… 📦 Distributed by @Coriell_Science & @Addgene #OpenScience
Plasmids for mStayGold E138D are now available through @Addgene. Link: addgene.org/search/catalog… @SPOREMOHAN @midbody @wtsctt @MairtMca
14. Get your SOXmas presents @Addgene addgene.org/browse/article…
Most of our pHYX plasmids recently published doi.org/10.1016/j.ymbe… are now available @Addgene (addgene.org/browse/article…). And merry Christmas to all! 🎅🎄 @ECCP_lab
We've previously reported PAM variable PEmax for correcting KRAS mutations, and now our constructs - PEmax-SpG, PEmax-SpG-P2A-hMLH1dn - are available on @Addgene! Check them out! #CRISPR #PrimeEditing addgene.org/browse/article…
Troponin I (slow skeletal) is our #CellLineOfTheWeek! ❤️🔬 Our human iPS cell lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCel… 📦 Distributed by @Coriell_Science & @Addgene. #OpenScience
If you liked my thread about mStayGold variants, it found a more permanent home @focalplane_jcs, with updated @Addgene links: focalplane.biologists.com/2023/12/19/sta…
If you liked my thread about mStayGold variants, it found a more permanent home @focalplane_jcs, with updated @Addgene links: focalplane.biologists.com/2023/12/19/sta… https://t.co/zidN0og9bf
Perspective (OA): "The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene" liebertpub.com/doi/10.1089/cr… >15,000 plasmids from >1,000 laboratories ∼40 countries 300,000 shipments to ∼5,000 organizations in ∼100 countries @Addgene @CRISPRchef
Haemophilus influenzae was the first bacteria to be sequenced but few genetic tools exist for this organism. It has small genome of only 1.8 Mbp and can be easily grown in lab. To speed up synbio research for this organism we created plasmids that now you can get @Addgene
We're depositing parts on @Addgene and hope that our tools join the MoClo-YTK ecosystem to make yeast synbio more accessible, alongside recent work from @willmshaw, @MoKhalilLab, and @ProfTomEllis (pubs.acs.org/doi/10.1021/ac…), whose previous tools have enabled a lot of our work.
You can find us on: Instagram: hubs.la/Q02cTfDN0 TikTok: hubs.la/Q02cT7wG0 Facebook: hubs.la/Q02cT7pp0 LinkedIn: hubs.la/Q02cTctc0 Addgene Blog: hubs.la/Q02cT8570
‼️‼️ BioE3 and SUMO-ID plasmids now available at @Addgene . Thank you Addgene for your amazing job! 👇👇👇 addgene.org/Rosa_Barrio/
‼️‼️ BioE3 and SUMO-ID plasmids now available at @Addgene . Thank you Addgene for your amazing job! 👇👇👇 addgene.org/Rosa_Barrio/ https://t.co/MXrgpG69EY
Click editor plasmids now available on @Addgene addgene.org/browse/article… 🧬🖱️ Looking forward to feedback from the community on how well click editing works in your hands! @FerreiraSilvaJ @connorjtou @EmilyM_King @Madeline_Eller_ @Linyuan79834832 @DavidRufinoRam1
Click editor plasmids now available on @Addgene addgene.org/browse/article… 🧬🖱️ Looking forward to feedback from the community on how well click editing works in your hands! @FerreiraSilvaJ @connorjtou @EmilyM_King @Madeline_Eller_ @Linyuan79834832 @DavidRufinoRam1
Addgene has shared THOUSANDS of plasmids with the scientific community on behalf of the Looger Lab (@LoogerL) - the blue flames you see on their @Addgene page are a testament to how much people like the tools they generate! addgene.org/Loren_Looger/
Addgene has shared THOUSANDS of plasmids with the scientific community on behalf of the Looger Lab (@LoogerL) - the blue flames you see on their @Addgene page are a testament to how much people like the tools they generate! addgene.org/Loren_Looger/
The plasmids are available on @Addgene, including recombinases, reporters, and empty vectors for your own favorite gene: addgene.org/browse/article…
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662 Followers 476 Following Virologist | Influenza | Immunity | Polymerases | RNA Viruses | PostDoc @institutpasteur #naffakhlab | Tweets for science 🇪🇺
Borris Aldonza (보�.. @PrecursorCell
1K Followers 2K Following Evolution, animals, stem cells, glycans, tumor ecology. PhDing @SeoulNatlUni. Prev @KAISTPR @IMBA_Vienna @UTokyo_News_en. Rev Editor @FrontCellDevBio 👨🔬🌈🐕
Anthony Kang @kanga_2roo
86 Followers 493 Following MD/MBA student @NUFeinbergMed/@KelloggSchool | synbio, tidyverse, taco tuesday
(although I suspect that this $85 fee doesn't actually cover the costs and that @Addgene are just being super-cool about it to be able to serve the international community). Thank you, Addgene, you excellent people. 11/12
🎊 Happy birthday, @Addgene! 🥳 Today we cell-ebrate you and the 137,289 plasmids you have sent on behalf of 5,822 labs from around the world!🤯 Get to know Addgene's #OpenScience mission: addgene.org
Our Cell Line of the Week is SERCA2 - illuminating the sarcoplasmic/ endoplasmic reticulum! 💪 Browse our openly available hiPSC lines & plasmids – fluorescently-tagged to illuminate structures: bit.ly/AccessAllenCel… 📦 Distributed by @Coriell_Science & @Addgene
We hope that the GSH will have applications in #stemcell research and potentially in #celltherapy and we will make reagents available via @WiCellResearch and @Addgene. Please get in touch if you have questions on our work. 3/n
The key reagents from our recent CombiCell manuscript are now available on @Addgene addgene.org/Omer_Dushek/ @embojournal embopress.org/doi/full/10.10…
Our new work! Using CombiCells, a platform enabling titration and combinatorial display of cell surface ligands, to investigate the sensitivity and costimulatory requirements of TCRs and CARs biorxiv.org/content/10.110…
Working with @Addgene to make all plasmids easy to get soon
Our #CellLineOfTheWeek is ras-related protein - targeting endosomes! 🧫 Our hiPSC lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCel… 📦 Distributed by @Coriell_Science & @Addgene #OpenScience
Plasmids for mStayGold E138D are now available through @Addgene. Link: addgene.org/search/catalog… @SPOREMOHAN @midbody @wtsctt @MairtMca
14. Get your SOXmas presents @Addgene addgene.org/browse/article…
Most of our pHYX plasmids recently published doi.org/10.1016/j.ymbe… are now available @Addgene (addgene.org/browse/article…). And merry Christmas to all! 🎅🎄 @ECCP_lab
We've previously reported PAM variable PEmax for correcting KRAS mutations, and now our constructs - PEmax-SpG, PEmax-SpG-P2A-hMLH1dn - are available on @Addgene! Check them out! #CRISPR #PrimeEditing addgene.org/browse/article…
Troponin I (slow skeletal) is our #CellLineOfTheWeek! ❤️🔬 Our human iPS cell lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCel… 📦 Distributed by @Coriell_Science & @Addgene. #OpenScience
If you liked my thread about mStayGold variants, it found a more permanent home @focalplane_jcs, with updated @Addgene links: focalplane.biologists.com/2023/12/19/sta…
Lost in the StayGold monomeric variants? Here's a thread to try to sort things out 🧵 In 2022, the Katayama/Miyawaki labs published StayGold, a bright and stable fluorescent protein (FP) from jellyfish C. Uchidae: nature.com/articles/s4158… @NatureBiotech
Perspective (OA): "The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene" liebertpub.com/doi/10.1089/cr… >15,000 plasmids from >1,000 laboratories ∼40 countries 300,000 shipments to ∼5,000 organizations in ∼100 countries @Addgene @CRISPRchef
Browsing @Addgene as a synthetic biologist is like a trip to the merchant in Zelda games. You want everything even if you have no idea how you might use it just yet. “The shadow tyrosine kinase of the winds” - sounds important.
Plasmid kits for the Ubiquiton technology will be available via @Addgene soon.
Haemophilus influenzae was the first bacteria to be sequenced but few genetic tools exist for this organism. It has small genome of only 1.8 Mbp and can be easily grown in lab. To speed up synbio research for this organism we created plasmids that now you can get @Addgene
We're depositing parts on @Addgene and hope that our tools join the MoClo-YTK ecosystem to make yeast synbio more accessible, alongside recent work from @willmshaw, @MoKhalilLab, and @ProfTomEllis (pubs.acs.org/doi/10.1021/ac…), whose previous tools have enabled a lot of our work.
We created a suite of attB backbone vectors (Multisite Gateway, restriction sites) with and without transgenesis markers, primer info for quality control, & detailed protocols.⭕️🧫 All vectors are being deposited with @Addgene and available from us! #ShareYourTools 12/n
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